We developed a near infrared fluorophore-conjugated anti–Carcinoembryonic antigen (CEA) antibody for mice bearing tumor of CEA expressing cells, and demonstrated in vivo optical imaging by macro zoom microscopy. In the result, tumors of CEA-expressing cancer cells were specifically detected in vivo. Furthermore, cancer-specific fluorescence images were acquired at subcellular level in vivo by two-photon microscopy. In preclinical applications, the lymph node micrometastasis was also successfully visualized by two-photon microscopy. These results suggest that two-photon excitation microscopy in combination with an immunoconjugated probe could be widely adapted to cancer detection in clinical settings.

Date Published: 12 March 2015
PDF: 4 pages
Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390J (12 March 2015); doi: 10.1117/12.2079092

Published in SPIE Proceedings Vol. 9339:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII
Samuel Achilefu; Ramesh Raghavachari, Editor(s)