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Proceedings Paper

Non-linear imaging and characterization of atherosclerotic arterial tissue using combined SHG and FLIM microscopy
Author(s): Riccardo Cicchi; Christian Matthäus; Tobias Meyer; Annika Lattermann; Benjamin Dietzek; Bernhard R. Brehm; Jürgen Popp; Francesco S. Pavone
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Paper Abstract

Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view, especially for what is concerning collagen content and organization because collagen plays a crucial role in plaque vulnerability. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires immune-histochemical examination and a morpho-functional approach. Non-linear microscopy techniques offer the potential for providing morpho-functional information on the examined tissues in a label-free way. In this study, we employed combined SHG and FLIM microscopy for characterizing collagen organization in both normal arterial wall and within atherosclerotic plaques. Image pattern analysis of SHG images allowed characterizing collagen organization in different tissue regions. In addition, the analysis of collagen fluorescence decay contributed to the characterization of the samples on the basis of collagen fluorescence lifetime. Different values of collagen fiber mean size, collagen distribution, collagen anisotropy and collagen fluorescence lifetime were found in normal arterial wall and within plaque depositions, prospectively allowing for automated classification of atherosclerotic lesions and plaque vulnerability. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue and has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

Paper Details

Date Published: 5 March 2015
PDF: 4 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932919 (5 March 2015); doi: 10.1117/12.2078892
Show Author Affiliations
Riccardo Cicchi, National Insitute of Optics, CNR (Italy)
European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
Christian Matthäus, Institute of Photonic Technology (Germany)
Tobias Meyer, Institute of Photonic Technology (Germany)
Annika Lattermann, Friedrich Schiller Univ. (Germany)
Benjamin Dietzek, Institute of Photonic Technology (Germany)
Friedrich Schiller Univ. (Germany)
Bernhard R. Brehm, Katholische Klinikum Koblenz (Germany)
Jürgen Popp, Institute of Photonic Technology (Germany)
Friedrich Schiller Univ. (Germany)
Francesco S. Pavone, National Institute of Optics, National Research Council (Italy)
European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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