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Proceedings Paper

Multiphoton tomography of astronauts
Author(s): Karsten König; Martin Weinigel; Anna Pietruszka; Rainer Bückle; Nicole Gerlach; Ulrike Heinrich
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Paper Abstract

Weightlessness may impair the astronaut’s health conditions. Skin impairments belong to the most frequent health problems during space missions. Within the Skin B project, skin physiological changes during long duration space flights are currently investigated on three European astronauts that work for nearly half a year at the ISS.

Measurements on the hydration, the transepidermal water loss, the surface structure, elasticity and the tissue density by ultrasound are conducted. Furthermore, high-resolution in vivo histology is performed by multiphoton tomography with 300 nm spatial and 200 ps temporal resolution. The mobile certified medical tomograph with a flexible 360° scan head attached to a mechano-optical arm is employed to measure two-photon autofluorescence and SHG in the volar forearm of the astronauts. Modification of the tissue architecture and of the fluorescent biomolecules NAD(P)H, keratin, melanin and elastin are detected as well as of SHG-active collagen. Thinning of the vital epidermis, a decrease of the autofluoresence intensity, an increase in the long fluorescence lifetime, and a reduced skin ageing index SAAID based on an increased collagen level in the upper dermis have been found. Current studies focus on recovery effects.

Paper Details

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290Q (5 March 2015); doi: 10.1117/12.2078823
Show Author Affiliations
Karsten König, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)
Martin Weinigel, JenLab GmbH (Germany)
Anna Pietruszka, JenLab GmbH (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Nicole Gerlach, DermaTronnier GmbH & Co. KG (Germany)
Ulrike Heinrich, DermaTronnier GmbH & Co. KG (Germany)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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