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Proceedings Paper

Software-aided automatic cell optoporation system
Author(s): Hans Georg Breunig; Ana Batista; Aisada Uchugonova; Karsten König
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Paper Abstract

Optoporation, the laser-induced transient cell membrane perforation, has emerged as a powerful non-invasive and highly efficient cell-transfection technique. It is usually done by targeting individual cells manually which significantly limits number of addressable cells. We present an experimental setup with custom-made software control for automated cell optoporation experiments. The automated software evaluates contrast edges in bright-field sample images to identify cell locations for laser illumination and controls the hardware to transiently laser-illuminate these positions. By software controlled stitching together of several microscopic field-of-views in principle all cells in a culture dish can be targeted without further user interaction. The software-based automation allows to significantly increase the number of treatable cells compared to a manual approach. We illustrate the experimental capabilities in CHO-cell optoporation experiments with highly focused beam-shaped sub-15 fs pulses.

Paper Details

Date Published: 2 March 2015
PDF: 7 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280D (2 March 2015); doi: 10.1117/12.2078728
Show Author Affiliations
Hans Georg Breunig, Saarland Univ. (Germany)
JenLab GmbH (Germany)
Ana Batista, Saarland Univ. (Germany)
Aisada Uchugonova, Saarland Univ. (Germany)
JenLab GmbH (Germany)
Karsten König, Saarland Univ. (Germany)
JenLab GmbH (Germany)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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