We present a method to reduce the inherent errors caused by band-pass filter in off-axis quantitative phase microscopy and propose the optimum condition that can minimize these errors. We found that phase information of a sample in frequency domain nonlinearly oscillates as a function of the phase-shift correspond to the sample and its medium and the phase information of a sample inside the band-pass filter can be maximized by a proper phase-shift. Through numerical simulations and actual experiments, we demonstrate that the error in phase volume measurement can be effectively reduced by the enhancement of phase signal inside band-pass region using an optimum amount of phase that can be controlled by either changing medium index or wavelength of illumination.

Date Published: 10 March 2015
PDF: 11 pages
Proc. SPIE 9386, Practical Holography XXIX: Materials and Applications, 93860P (10 March 2015); doi: 10.1117/12.2078681

Published in SPIE Proceedings Vol. 9386:
Practical Holography XXIX: Materials and Applications
Hans I. Bjelkhagen; V. Michael Bove Jr., Editor(s)