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Proceedings Paper

Label-free monitoring of individual DNA hybridization using SERS
Author(s): Ji Qi; Jianbo Zeng; Fusheng Zhao; Greggy M. Santos; Steven Hsesheng Lin; Balakrishnan Raja; Ulrich Strych; Richard C. Willson; Wei-Chuan Shih
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Paper Abstract

Sequence-specific detection of DNA hybridization at the single-molecule level has been instrumental and gradually become a ubiquitous tool in a wide variety of biological and biomedical applications such as clinical diagnostics, biosensors, and drug development. Label-free and amplification-free schemes are of particular interest because they could potentially provide in situ monitoring of individual hybridization events, which may lead to techniques for discriminating subtle variations due to single-base modification without stringency control or repetitive thermal cycling. Surface-enhanced Raman spectroscopy (SERS) has been widely used for molecular detection and identification by exploiting the localized surface plasmon resonance effect when the target molecules are near gold or silver nanostructures. However, effective and robust SERS assays have yet become a reality for trace detection. Recently, we have developed a SERS substrate by shaping nanoporous gold thin films into monolithic submicron disks, called nanoporous gold disks (NPGD). Here we demonstrate in situ monitoring of the same immobilized ssDNA molecules and their individual hybridization events.

Paper Details

Date Published: 11 March 2015
PDF: 5 pages
Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 93370D (11 March 2015); doi: 10.1117/12.2078552
Show Author Affiliations
Ji Qi, Univ. of Houston (United States)
Jianbo Zeng, Univ. of Houston (United States)
Fusheng Zhao, Univ. of Houston (United States)
Greggy M. Santos, Univ. of Houston (United States)
Steven Hsesheng Lin, MD Anderson Cancer Ctr. (United States)
Balakrishnan Raja, Univ. of Houston (United States)
Ulrich Strych, Univ. of Houston (United States)
Richard C. Willson, Univ. of Houston (United States)
Tecnológico de Monterey (Mexico)
Wei-Chuan Shih, Univ. of Houston (United States)

Published in SPIE Proceedings Vol. 9337:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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