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Proceedings Paper

Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control
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Paper Abstract

High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. In this paper, we combine these two methods to realize three-dimensional high-resolution imaging. Spatial light modulator (SLM) is used as a flexible phase mask of the microscopy. Multiple super-resolution focuses as an array or in arbitrary positions could be generated by phase retrieval. This microscopy by SLM control could applied to parallel two-photon RESOLFT imaging or multiple spots tracking in high-resolution.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932927 (5 March 2015); doi: 10.1117/12.2078509
Show Author Affiliations
Yi Xue, Massachusetts Institute of Technology (United States)
Christopher J. Rowlands, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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