
Proceedings Paper
Cytotoxicity change with albumin binding of talaporfin sodium in extracellular photosensitization reaction on cardiomyocyteFormat | Member Price | Non-Member Price |
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Paper Abstract
We studied the myocardial cell necrosis measuring cell lethality with various albumin concentrations against oxidative
stress by extracellular photosensitization reaction using talaporfin sodium. We have proposed to induce the
photosensitization reaction in interstitial space of myocardium with short drug-light interval to realize the immediate cell
damage by myocardial cell membrane for tachyarrhythmia treatment. To understand the effect of the particular
extracellular photosensitization reaction in interstitial space, we measured the myocardial cell lethality 2 hours after the
photosensitization reaction by WST assay varying the talaporfin sodium concentration, radiant exposure, and wide
albumin concentration of 0-15 mg/ml. The cell lethality was decreased with albumin concentration increasing over 85 %
in binding ratio between talaporfin sodium and albumin. The cell lethality didn’t change between 0-85% in binding ratio
between talaporfin sodium and albumin. The calculated deposited energy to the talaporfin sodium solution was nearly
constant of 8.4±1.6 J/well in average varying albumin concentration. We think the cell killing effect by the extracellular
photosensitization reaction has a threshold between 85-100% in the binding ratio between talaporfin sodium and albumin
in the case of the talaporfin sodium concentration of 40 μg/ml and radiant exposure of 0-40 J/cm2.
Paper Details
Date Published: 13 March 2015
PDF: 4 pages
Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210I (13 March 2015); doi: 10.1117/12.2078454
Published in SPIE Proceedings Vol. 9321:
Optical Interactions with Tissue and Cells XXVI
E. Duco Jansen, Editor(s)
PDF: 4 pages
Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210I (13 March 2015); doi: 10.1117/12.2078454
Show Author Affiliations
Tsunenori Arai, Keio Univ. (Japan)
Published in SPIE Proceedings Vol. 9321:
Optical Interactions with Tissue and Cells XXVI
E. Duco Jansen, Editor(s)
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