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Proceedings Paper

Analysis of bacteria-derived outer membrane vesicles using tunable resistive pulse sensing
Author(s): Evgeny Bogomolny; Jiwon Hong; Cherie Blenkiron; Denis Simonov; Priscila Dauros; Simon Swift; Anthony Phillips; Geoff R. Willmott
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Paper Abstract

Accurate characterization of submicron particles within biological fluids presents a major challenge for a wide range of biomedical research. Detection, characterization and classification are difficult due to the presence of particles and debris ranging from single molecules up to particles slightly smaller than cells. Especial interest arises from extracellular vesicles (EVs) which are known to play a pivotal role in cell-signaling in multicellular organisms. Tunable resistive pulse sensing (TRPS) is increasingly proving to be a useful tool for high throughput particle-by-particle analysis of EVs and other submicron particles. This study examines the capability of TRPS for characterization of EVs derived from bacteria, also called outer membrane vesicles (OMVs). Measurement of a size distribution (124 ± 3 nm modal diameter) and concentration (lower bound 7.4 x 109 mL-1) are demonstrated using OMVs derived from uropathogenic Escherichia coli. Important aspects of measurement are discussed, including sample preparation and size selection. Application of TRPS to study EVs could assist the development of these particles in clinical diagnostics and therapeutics.

Paper Details

Date Published: 12 March 2015
PDF: 6 pages
Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 93381K (12 March 2015); doi: 10.1117/12.2078377
Show Author Affiliations
Evgeny Bogomolny, The Univ. of Auckland (New Zealand)
Jiwon Hong, The Univ. of Auckland (New Zealand)
Cherie Blenkiron, The Univ. of Auckland (New Zealand)
Denis Simonov, The Univ. of Auckland (New Zealand)
Priscila Dauros, The Univ. of Auckland (New Zealand)
Simon Swift, The Univ. of Auckland (New Zealand)
Anthony Phillips, The Univ. of Auckland (New Zealand)
Geoff R. Willmott, The Univ. of Auckland (New Zealand)
The MacDiarmid Institute for Advanced Materials and Nanotechnology (New Zealand)

Published in SPIE Proceedings Vol. 9338:
Colloidal Nanoparticles for Biomedical Applications X
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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