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Proceedings Paper

Comparison of optical projection tomography and optical coherence tomography for assessment of murine embryonic development
Author(s): Manmohan Singh; Achuth Nair; Tegy Vadakkan; Victor Piazza; Ryan Udan; Michael V. Frazier; Trevor Janecek; Mary E. Dickinson; Kirill V. Larin
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Paper Abstract

The murine model is a common model for studying developmental diseases. In this study, we compare the performance of the relatively new method of Optical Projection Tomography (OPT) to the well-established technique of Optical Coherence Tomography (OCT) to assess murine embryonic development at three stages, 9.5, 11.5, and 13.5 days post conception. While both methods can provide spatial resolution at the micrometer scale, OPT can provide superior imaging depth compared to OCT. However, OPT requires samples to be fixed, placed in an immobilization media such as agar, and cleared before imaging. Because OCT does not require fixing, it can be used to image embryos in vivo and in utero. In this study, we compare the efficacy of OPT and OCT for imaging murine embryonic development. The data demonstrate the superior capability of OPT for imaging fine structures with high resolution in optically-cleared embryos while only OCT can provide structural and functional imaging of live embryos ex vivo and in utero with micrometer scale resolution.

Paper Details

Date Published: 10 March 2015
PDF: 8 pages
Proc. SPIE 9334, Optical Methods in Developmental Biology III, 93340J (10 March 2015); doi: 10.1117/12.2078319
Show Author Affiliations
Manmohan Singh, Univ. of Houston (United States)
Achuth Nair, Univ. of Houston (United States)
Tegy Vadakkan, Baylor College of Medicine (United States)
Victor Piazza, Baylor College of Medicine (United States)
Ryan Udan, Baylor College of Medicine (United States)
Michael V. Frazier, Univ. of Houston (United States)
Trevor Janecek, Univ. of Houston (United States)
Mary E. Dickinson, Baylor College of Medicine (United States)
Kirill V. Larin, Univ. of Houston (United States)
Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 9334:
Optical Methods in Developmental Biology III
Andrew M. Rollins; Scott E. Fraser; Michael A. Choma M.D., Editor(s)

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