The continuing advancement of nonlinear optical imaging techniques has opened many new windows in biological exploration. In this work, the nonlinear optical microscopy, based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), was extended to probe tumor response to preoperative radiochemotherapy (RCT) for rectal carcinoma. It was found that MPM has the ability of direct visualization of histopathologic changes in rectal carcinoma following preoperative RCT including stromal fibrosis, colloid response and residual tumors. Our results also showed the capability of MPM using the quantitative analyses of images to quantify these changes. This work may provide the groundwork for further exploration into the application of multiphoton-based endoscopy in a clinical setting.

Date Published: 9 March 2015
PDF: 7 pages
Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240R (9 March 2015); doi: 10.1117/12.2078055

Published in SPIE Proceedings Vol. 9324:
Biophotonics and Immune Responses X
Wei R. Chen, Editor(s)