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Proceedings Paper

External stimulation by nanosecond pulsed electric fields to enhance cellular uptake of nanoparticles
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Paper Abstract

As an increasing number of studies use gold nanoparticles (AuNPs) for potential medicinal, biosensing and therapeutic applications, the synthesis and use of readily functional, bio-compatible nanoparticles is receiving much interest. For these efforts, the particles are often taken up by the cells to allow for optimum sensing or therapeutic measures. This process typically requires incubation of the particles with the cells for an extended period. In an attempt to shorten and control this incubation, we investigated whether nanosecond pulsed electric field (nsPEF) exposure of cells will cause a controlled uptake of the particles. NsPEF are known to induce the formation of nanopores in the plasma membrane, so we hypothesized that by controlling the number, amplitude or duration of the nsPEF exposure, we could control the size of the nanopores, and thus control the particle uptake. Chinese hamster ovary (CHO-K1) cells were incubated sub-10 nm AuNPs with and without exposure to 600-ns electrical pulses. Contrary to our hypothesis, the nsPEF exposure was found to actually decrease the particle uptake in the exposed cells. This result suggests that the nsPEF exposure may be affecting the endocytotic pathway and processes due to membrane disruption.

Paper Details

Date Published: 13 March 2015
PDF: 7 pages
Proc. SPIE 9326, Energy-based Treatment of Tissue and Assessment VIII, 932610 (13 March 2015); doi: 10.1117/12.2077950
Show Author Affiliations
Samantha Franklin, The Univ. of Texas at San Antonio (United States)
Air Force Research Lab. (United States)
Hope T. Beier, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Kelly Nash, The Univ. of Texas at San Antonio (United States)

Published in SPIE Proceedings Vol. 9326:
Energy-based Treatment of Tissue and Assessment VIII
Thomas P. Ryan, Editor(s)

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