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Proceedings Paper

Transferability of antibody pairs from ELISA to fiber optic surface plasmon resonance for infliximab detection
Author(s): Thomas Van Stappen; Jiadi Lu; Maarten Bloemen; Nick Geukens; Dragana Spasic; Filip Delport; Thierry Verbiest; Jeroen Lammertyn; Ann Gils
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Paper Abstract

Tumor necrosis factor (TNF)-alpha is a pleiotropic cytokine up-regulated in inflammatory bowel disease, rheumatoid arthritis and psoriasis. The introduction of anti-TNF drugs such as infliximab has revolutionized the treatment of these diseases. Recently, therapeutic drug monitoring (TDM) of infliximab has been introduced in clinical decision making to increase cost-efficiency. Nowadays, TDM is performed using radio-immunoassays, homogeneous mobility shift assays or ELISA. Unfortunately, these assays do not allow for in situ treatment optimization, because of the required sample transportation to centralized laboratories and the subsequent assay execution time. In this perspective, we evaluated the potential of fiber optic-surface plasmon resonance (FO-SPR). To achieve this goal, a panel of 55 monoclonal anti-infliximab antibodies (MA-IFX) was developed and characterized in-house, leading to the identification of nine different clusters. Based on this high diversity, 22 antibody pairs were selected and tested for their reactivity towards IFX, using one MA-IFX as capture and one MA-IFX for detection, in a sandwich type ELISA and FO-SPR. This study showed that the reactivity towards IFX of each antibody pair in ELISA is highly similar to its reactivity on FO-SPR, indicating that antibody pairs are easily transferable between both platforms. Given the fact that FO-SPR shows the potential for miniaturization and fast assay time, it can be considered a highly promising platform for on-site infliximab monitoring.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9317, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XV, 931705 (5 March 2015); doi: 10.1117/12.2077684
Show Author Affiliations
Thomas Van Stappen, KU Leuven (Belgium)
Jiadi Lu, Katholieke Univ. Leuven (Belgium)
Maarten Bloemen, KU Leuven (Belgium)
Nick Geukens, KU Leuven (Belgium)
Dragana Spasic, KU Leuven (Belgium)
Filip Delport, Katholieke Univ. Leuven (Belgium)
Thierry Verbiest, KU Leuven (Belgium)
Jeroen Lammertyn, KU Leuven (Belgium)
Ann Gils, KU Leuven (Belgium)

Published in SPIE Proceedings Vol. 9317:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XV
Israel Gannot, Editor(s)

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