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Proceedings Paper

Development of a DMD-based fluorescence microscope
Author(s): Nadya Chakrova; Bernd Rieger; Sjoerd Stallinga
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Paper Abstract

We present a versatile fluorescence microscope, built by complementing a conventional fluorescence microscope with a digital micro-mirror device (DMD) in the illumination path. Arbitrary patterns can be created on the DMD and projected onto the sample. This patterned illumination can be used to improve lateral and axial resolution over the resolution of a wide-field microscope, as well as to reduce the illumination dose. Different illumination patterns require different reconstruction strategies and result in an image quality similar to confocal or structured illumination microscopy. We focus on the optical design and characterization of a DMD-based microscope. Estimation of the optical quality of the microscope has been carried out by measuring the modulation transfer function from edge profiles. We have obtained optically sectioned images by applying multi-spot illumination patterns followed by digital pinholing. The sectioning capabilities of our DMD-based microscope were estimated from the dependence of the signal-to-background and signalto-noise ratios on the pitch of the projected multi-spot patterns and the size of the digital pinhole. In addition, we provide an outlook on the use of pseudo-random illumination patterns for achieving both sectioning and resolution enhancement.

Paper Details

Date Published: 9 March 2015
PDF: 11 pages
Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 933008 (9 March 2015); doi: 10.1117/12.2077677
Show Author Affiliations
Nadya Chakrova, Delft Univ. of Technology (Netherlands)
Bernd Rieger, Technische Univ. Delft (Netherlands)
Sjoerd Stallinga, Delft Univ. of Technology (Netherlands)

Published in SPIE Proceedings Vol. 9330:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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