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Proceedings Paper

Development of a multi-scale and multi-modality imaging system to characterize tumours and their microenvironment in vivo
Author(s): Valérie Rouffiac; Karine Ser-Leroux; Emilie Dugon; Ingrid Leguerney; Mélanie Polrot; Sandra Robin; Sophie Salomé-Desnoulez; Jean-Christophe Ginefri; Catherine Sebrié; Corinne Laplace-Builhé
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Paper Abstract

In vivo high-resolution imaging of tumor development is possible through dorsal skinfold chamber implantable on mice model. However, current intravital imaging systems are weakly tolerated along time by mice and do not allow multimodality imaging. Our project aims to develop a new chamber for: 1- long-term micro/macroscopic visualization of tumor (vascular and cellular compartments) and tissue microenvironment; and 2- multimodality imaging (photonic, MRI and sonography). Our new experimental device was patented in March 2014 and was primarily assessed on 75 mouse engrafted with 4T1-Luc tumor cell line, and validated in confocal and multiphoton imaging after staining the mice vasculature using Dextran 155KDa-TRITC or Dextran 2000kDa-FITC. Simultaneously, a universal stage was designed for optimal removal of respiratory and cardiac artifacts during microscopy assays. Experimental results from optical, ultrasound (Bmode and pulse subtraction mode) and MRI imaging (anatomic sequences) showed that our patented design, unlike commercial devices, improves longitudinal monitoring over several weeks (35 days on average against 12 for the commercial chamber) and allows for a better characterization of the early and late tissue alterations due to tumour development. We also demonstrated the compatibility for multimodality imaging and the increase of mice survival was by a factor of 2.9, with our new skinfold chamber. Current developments include: 1- defining new procedures for multi-labelling of cells and tissue (screening of fluorescent molecules and imaging protocols); 2- developing ultrasound and MRI imaging procedures with specific probes; 3- correlating optical/ultrasound/MRI data for a complete mapping of tumour development and microenvironment.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9316, Multimodal Biomedical Imaging X, 93160I (5 March 2015); doi: 10.1117/12.2077653
Show Author Affiliations
Valérie Rouffiac, Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS (France)
Karine Ser-Leroux, Institut Gustave Roussy (France)
Emilie Dugon, Institut Gustave Roussy (France)
Ingrid Leguerney, Univ. Paris-Sud, CNRS (France)
Mélanie Polrot, Institut Gustave Roussy (France)
Sandra Robin, Univ. Paris-Sud, CNRS (France)
Sophie Salomé-Desnoulez, Institut Gustave Roussy (France)
Jean-Christophe Ginefri, Univ. Paris-Sud, CNRS (France)
Catherine Sebrié, Univ. Paris-Sud, CNRS (France)
Corinne Laplace-Builhé, Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS (France)

Published in SPIE Proceedings Vol. 9316:
Multimodal Biomedical Imaging X
Fred S. Azar; Xavier Intes, Editor(s)

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