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Proceedings Paper

Clinical approved fluorescent dyes coupled to endomicroscopy for in vivo diagnostic of peritoneal carcinomatosis
Author(s): Muriel Abbaci; Peggy Dartigues; Ranya Soufan; Frederic De Leeuw; Monique Fabre M.D.; Corinne Laplace-Builhé
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Paper Abstract

Peritoneal carcinomatosis is metastatic stage aggravating digestive, gynecological or bladder cancer dissemination and the preoperative evaluation of lesions remains difficult. There is therefore a need for minimal invasive innovative techniques to establish a precise preoperative assessment of cancer peritoneal cavity. Probe-based confocal laser endomicroscopy (pCLE) provides dynamic images of the microarchitecture of tissues during an endoscopy. The PERSEE project proposes new developments in robotics and pCLE for the exploration of the peritoneal cavity during laparoscopy. Two fluorescent dyes, Patent blue V and Indocyanine green have been evaluated on human ex vivo samples to improve the contrast of pCLE images. For a future implementation in clinical study, two topically staining protocols operable in vivo have been validated on 70 specimens from 25 patients with a peritoneal carcinomatosis. The specimens were then imaged by pCLE with an optical probe designed for the application. A histo-morphological correlative study was performed on 350 pCLE images and 70 standard histological preparations. All images were interpreted in a random way by two pathologists. Differential histological diagnostics such as normal peritoneum or pseudomyxoma could be recognized on fluorescence images. The statistical analysis of the correlative study is underway. These dyes already approved for human use are interesting for pCLE imaging because some micromorphological criteria look like to conventional histology and are readable by pathologist. Thus pCLE images using both dyes do not require a specific semiology unlike to what is described in the literature, for pCLE associated with fluorescein for the in vivo imaging of pancreatic cysts.

Paper Details

Date Published: 2 March 2015
PDF: 7 pages
Proc. SPIE 9304, Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II, 93040R (2 March 2015); doi: 10.1117/12.2077650
Show Author Affiliations
Muriel Abbaci, Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS, IR4M (France)
Peggy Dartigues, Institut Gustave Roussy (France)
Ranya Soufan, Institut Gustave Roussy (France)
Frederic De Leeuw, Institut Gustave Roussy (France)
Monique Fabre M.D., Institut Gustave Roussy (France)
Univ. Paris-Sud, CNRS, IR4M (France)
Corinne Laplace-Builhé, Institut Gustave Roussy (France)

Published in SPIE Proceedings Vol. 9304:
Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II
Melissa J. Suter; Guillermo J. Tearney M.D.; Thomas D. Wang; Stephen Lam M.D.; Matthew Brenner, Editor(s)

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