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Proceedings Paper

The effect of UV-Vis to near-infrared light on the biological response of human dental pulp cells
Author(s): Mohammed A. Hadis; Paul R. Cooper; Michael R. Milward; Patricia Gorecki; Edward Tarte; James Churm; William M. Palin
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Paper Abstract

Human dental pulp cells (DPCs) were isolated and cultured in phenol-red-free α-MEM/10%-FCS at 37ºC in 5% CO2. DPCs at passages 2-4 were seeded (150μL; 25,000 cell/ml) in black 96-microwell plates with transparent bases. 24h post-seeding, cultures were irradiated using a bespoke LED array consisting of 60 LEDs (3.5mW/cm2) of wavelengths from 400-900nm (10 wavelengths, n=6) for time intervals of up to 120s. Metabolic and mitochondrial activity was assessed via a modified MTT assay. Statistical differences were identified using multi-factorial analysis of variance and post-hoc Tukey tests (P=0.05). The biological responses were significantly dependent upon post-irradiation incubation period, wavelength and exposure time (P<0.05). At shorter wavelength irradiances (400nm), a reduction in mitochondrial activity was detected although not significant, whereas longer wavelength irradiances (at 633, 656, 781 and 799nm) significantly increased mitochondrial activity (P<0.05) in DPCs. At these wavelengths, mitochondrial activity was generally increased for exposures less than 90s with 30s exposures being most effective with 24h incubation. Increasing the post-irradiation incubation period increased the measured response and identified further significance (P<0.05). The biological responses of human DPCs were wavelength, exposure-time and incubation period dependent. The optimisation of irradiation parameters will be key to the successful application of LLLT in dentistry.

Paper Details

Date Published: 5 March 2015
PDF: 13 pages
Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 930906 (5 March 2015); doi: 10.1117/12.2077645
Show Author Affiliations
Mohammed A. Hadis, The Univ. of Birmingham (United Kingdom)
Paul R. Cooper, The Univ. of Birmingham (United Kingdom)
Michael R. Milward, The Univ. of Birmingham (United Kingdom)
Patricia Gorecki, The Univ. of Birmingham (United Kingdom)
Edward Tarte, The Univ. of Birmingham (United Kingdom)
James Churm, The Univ. of Birmingham (United Kingdom)
William M. Palin, The Univ. of Birmingham (United Kingdom)

Published in SPIE Proceedings Vol. 9309:
Mechanisms for Low-Light Therapy X
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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