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Proceedings Paper

Tryptophan as key biomarker to detect gastrointestinal tract cancer using non-negative biochemical analysis of native fluorescence and Stokes Shift spectroscopy
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Paper Abstract

The objective of this study was to find out the emission spectral fingerprints for discrimination of human colorectal and gastric cancer from normal tissue in vitro by applying native fluorescence. The native fluorescence (NFL) and Stokes shift spectra of seventy-two human cancerous and normal colorectal (colon, rectum) and gastric tissues were analyzed using three selected excitation wavelengths (e.g. 300 nm, 320 nm and 340 nm). Three distinct biomarkers, tryptophan, collagen and reduced nicotinamide adenine dinucleotide hydrate (NADH), were found in the samples of cancerous and normal tissues from eighteen subjects. The spectral profiles of tryptophan exhibited a sharp peak in cancerous colon tissues under a 300 nm excitation when compared with normal tissues. The changes in compositions of tryptophan, collagen, and NADH were found between colon cancer and normal tissues under an excitation of 300 nm by the non-negative basic biochemical component analysis (BBCA) model.

Paper Details

Date Published: 11 March 2015
PDF: 7 pages
Proc. SPIE 9318, Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 93180X (11 March 2015); doi: 10.1117/12.2077304
Show Author Affiliations
Leana Wang, New York Univ. (United States)
The City College of New York (United States)
Yan Zhou, The General Hospital of the Air Force, PLA (China)
Cheng-hui Liu, The City College of New York (United States)
Lixin Zhou, Beijing Cancer Hospital (China)
Yong He, Beijing Normal Univ. (China)
Yang Pu, The City College of New York (United States)
Thien An Nguyen, The City College of New York (United States)
Robert R. Alfano, The City College of New York (United States)


Published in SPIE Proceedings Vol. 9318:
Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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