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Proceedings Paper

Label-free structural photoacoustic tomography of intact mouse brain
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Paper Abstract

Capitalizing on endogenous hemoglobin contrast, photoacoustic computed tomography (PACT), a deep-tissue highresolution imaging modality, has drawn increasing interest in neuro-imaging. However, most existing studies are limited to functional imaging on the cortical surface, and the deep-brain structural imaging capability of PACT has never been demonstrated. Here, we explicitly studied the limiting factors of deep-brain PACT imaging. We found that the skull distorted the acoustic signal and blood suppressed the structural contrast from other chromophores. When the two effects are mitigated, PACT can provide high-resolution label-free structural imaging through the entire mouse brain. With 100 μm in-plane resolution, we can clearly identify major structures of the brain, and the image quality is comparable to that of magnetic resonance microscopy. Spectral PACT studies indicate that structural contrasts mainly originate from cytochrome and lipid. The feasibility of imaging the structure of the brain in vivo has also been discussed. Our results demonstrate that PACT is a promising modality for both structural and functional brain imaging.

Paper Details

Date Published: 11 March 2015
PDF: 11 pages
Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93230M (11 March 2015); doi: 10.1117/12.2077236
Show Author Affiliations
Lei Li, Washington Univ. in St. Louis (United States)
Jun Xia, Washington Univ. in St. Louis (United States)
Guo Li, Washington Univ. in St. Louis (United States)
Alejandro Garcia-Uribe, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 9323:
Photons Plus Ultrasound: Imaging and Sensing 2015
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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