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Proceedings Paper

Multilayered phantoms with tunable optical properties for a better understanding of light/tissue interactions
Author(s): Blandine Roig; Anne Koenig; François Perraut; Olivier Piot; Séverine Vignoud; Jonathan Lavaud; Michel Manfait; Jean-Marc Dinten
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Paper Abstract

Light/tissue interactions, like diffuse reflectance, endogenous fluorescence and Raman scattering, are a powerful means for providing skin diagnosis. Instrument calibration is an important step. We thus developed multilayered phantoms for calibration of optical systems. These phantoms mimic the optical properties of biological tissues such as skin. Our final objective is to better understand light/tissue interactions especially in the case of confocal Raman spectroscopy.

The phantom preparation procedure is described, including the employed method to obtain a stratified object. PDMS was chosen as the bulk material. TiO2 was used as light scattering agent. Dye and ink were adopted to mimic, respectively, oxy-hemoglobin and melanin absorption spectra. By varying the amount of the incorporated components, we created a material with tunable optical properties.

Monolayer and multilayered phantoms were designed to allow several characterization methods. Among them, we can name: X-ray tomography for structural information; Diffuse Reflectance Spectroscopy (DRS) with a homemade fibered bundle system for optical characterization; and Raman depth profiling with a commercial confocal Raman microscope for structural information and for our final objective.

For each technique, the obtained results are presented and correlated when possible.

A few words are said on our final objective. Raman depth profiles of the multilayered phantoms are distorted by elastic scattering. The signal attenuation through each single layer is directly dependent on its own scattering property. Therefore, determining the optical properties, obtained here with DRS, is crucial to properly correct Raman depth profiles. Thus, it would be permitted to consider quantitative studies on skin for drug permeation follow-up or hydration assessment, for instance.

Paper Details

Date Published: 6 March 2015
PDF: 6 pages
Proc. SPIE 9325, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue VII, 93250B (6 March 2015); doi: 10.1117/12.2077212
Show Author Affiliations
Blandine Roig, CEA, LETI (France)
Anne Koenig, CEA-LETI (France)
François Perraut, CEA, LETI (France)
Olivier Piot, MéDIAN Biophotonique et Technologies pour la Santé (France)
Séverine Vignoud, CEA, LETI (France)
Jonathan Lavaud, Institut Albert Bonniot (France)
Michel Manfait, MéDIAN Biophotonique et Technologies pour la Santé (France)
Jean-Marc Dinten, CEA, LETI (France)

Published in SPIE Proceedings Vol. 9325:
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue VII
David W. Allen; Jean-Pierre Bouchard, Editor(s)

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