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Proceedings Paper

Protein assay structured on paper by using lithography
Author(s): E. Wilhelm; T. M. Nargang; W. Al Bitar; B. Waterkotte; B. E. Rapp
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Paper Abstract

There are two main challenges in producing a robust, paper-based analytical device. The first one is to create a hydrophobic barrier which unlike the commonly used wax barriers does not break if the paper is bent. The second one is the creation of the (bio-)specific sensing layer. For this proteins have to be immobilized without diminishing their activity. We solve both problems using light-based fabrication methods that enable fast, efficient manufacturing of paper-based analytical devices. The first technique relies on silanization by which we create a flexible hydrophobic barrier made of dimethoxydimethylsilane. The second technique demonstrated within this paper uses photobleaching to immobilize proteins by means of maskless projection lithography. Both techniques have been tested on a classical lithography setup using printed toner masks and on a lithography system for maskless lithography. Using these setups we could demonstrate that the proposed manufacturing techniques can be carried out at low costs. The resolution of the paper-based analytical devices obtained with static masks was lower due to the lower mask resolution. Better results were obtained using advanced lithography equipment. By doing so we demonstrated, that our technique enables fabrication of effective hydrophobic boundary layers with a thickness of only 342 μm. Furthermore we showed that flourescine-5-biotin can be immobilized on the non-structured paper and be employed for the detection of streptavidinalkaline phosphatase. By carrying out this assay on a paper-based analytical device which had been structured using the silanization technique we proofed biological compatibility of the suggested patterning technique.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII, 932004 (5 March 2015); doi: 10.1117/12.2077136
Show Author Affiliations
E. Wilhelm, Karlsruher Institut für Technologie (Germany)
T. M. Nargang, Karlsruher Institut für Technologie (Germany)
W. Al Bitar, Karlsruher Institut für Technologie (Germany)
B. Waterkotte, Karlsruher Institut für Technologie (Germany)
B. E. Rapp, Karlsruher Institut für Technologie (Germany)

Published in SPIE Proceedings Vol. 9320:
Microfluidics, BioMEMS, and Medical Microsystems XIII
Bonnie L. Gray; Holger Becker, Editor(s)

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