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Proceedings Paper

Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots
Author(s): Lauren D. Field; Bertalan K. Andrásfalvy; Gregorio L. Galiñanes; Daniel Huber; Mladen Barbic; John J. Macklin; Kimihiro Susumu; James B. Delehanty III; Alan L. Huston; Judit K. Makara; Igor L. Medintz
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Paper Abstract

The simultaneous visualization, identification and targeting of neurons during patch clamp-mediated electrophysiological recordings is a basic technique in neuroscience, yet it is often complicated by the inability to visualize the pipette tip, particularly in deep brain tissue. Here we demonstrate a novel approach in which fluorescent quantum dot probes are used to coat pipettes prior to their use. The strong two-photon absorption cross sections of the quantum dots afford robust contrast at significantly deeper penetration depths than current methods allow. We demonstrate the utility of this technique in multiple recording formats both in vitro and in vivo where imaging of the pipettes is achieved at remarkable depths (up to 800 microns). Notably, minimal perturbation of cellular physiology is observed over the hours-long time course of neuronal recordings. We discuss our results within the context of the role that quantum dot nanoprobes may play in understanding neuronal cell physiology.

Paper Details

Date Published: 10 March 2015
PDF: 6 pages
Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93050Y (10 March 2015); doi: 10.1117/12.2076934
Show Author Affiliations
Lauren D. Field, U.S. Naval Research Lab. (United States)
Univ. of Maryland College Park (United States)
Bertalan K. Andrásfalvy, Hungarian Academy of Sciences (Hungary)
Gregorio L. Galiñanes, Univ. de Genève (Switzerland)
Daniel Huber, Univ. de Genève (Switzerland)
Mladen Barbic, Howard Hughes Medical Institute (United States)
John J. Macklin, Howard Hughes Medical Institute (United States)
Kimihiro Susumu, U.S. Naval Research Lab. (United States)
Sotera Defense Solutions (United States)
James B. Delehanty III, U.S. Naval Research Lab. (United States)
Alan L. Huston, U.S. Naval Research Lab. (United States)
Judit K. Makara, Institute of Experimental Medicine (Hungary)
Igor L. Medintz, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 9305:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Henry Hirschberg M.D.; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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