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Proceedings Paper

Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT
Author(s): Naoko Senda; Kentaro Osawa
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Paper Abstract

There is a need for development of non-invasive technique to evaluate regenerative tissues such as cell sheets for transplantation. We demonstrated non-invasive imaging inside living cell sheets of human oral mucosal epithelial cells by phase-diversity homodyne optical coherence tomography (OCT). The new method OCT developed in Hitachi enables cell imaging because of high resolution (axial resolution; ~2.6 μm, lateral resolution; ~1 μm, in the air). Nuclei inside cell sheets were imaged with sufficient spatial resolution to identify each cell. It suggested that the new method OCT could be useful for non-invasive cell sheet evaluation test.

Paper Details

Date Published: 2 March 2015
PDF: 5 pages
Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281E (2 March 2015); doi: 10.1117/12.2076762
Show Author Affiliations
Naoko Senda, Hitachi, Ltd. (Japan)
Kentaro Osawa, Hitachi, Ltd. (Japan)

Published in SPIE Proceedings Vol. 9328:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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