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Proceedings Paper

Fluorescence molecular imaging system with a novel mouse surface extraction method and a rotary scanning scheme
Author(s): Yue Zhao; Dianwen Zhu; Reheman Baikejiang; Changqing Li
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Paper Abstract

We have developed a new fluorescence molecular tomography (FMT) imaging system, in which we utilized a phase shifting method to extract the mouse surface geometry optically and a rotary laser scanning approach to excite fluorescence molecules and acquire fluorescent measurements on the whole mouse body. Nine fringe patterns with a phase shifting of 2π/9 are projected onto the mouse surface by a projector. The fringe patterns are captured using a webcam to calculate a phase map that is converted to the geometry of the mouse surface with our algorithms. We used a DigiWarp approach to warp a finite element mesh of a standard digital mouse to the measured mouse surface thus the tedious and time-consuming procedure from a point cloud to mesh is avoided. Experimental results indicated that the proposed method is accurate with errors less than 0.5 mm. In the FMT imaging system, the mouse is placed inside a conical mirror and scanned with a line pattern laser that is mounted on a rotation stage. After being reflected by the conical mirror, the emitted fluorescence photons travel through central hole of the rotation stage and the band pass filters in a motorized filter wheel, and are collected by a CCD camera. Phantom experimental results of the proposed new FMT imaging system can reconstruct the target accurately.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93190T (5 March 2015); doi: 10.1117/12.2076476
Show Author Affiliations
Yue Zhao, Univ. of California, Merced (United States)
Dianwen Zhu, Univ. of California, Merced (United States)
Reheman Baikejiang, Univ. of California, Merced (United States)
Changqing Li, Univ. of California, Merced (United States)

Published in SPIE Proceedings Vol. 9319:
Optical Tomography and Spectroscopy of Tissue XI
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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