We have designed and built a two-photon microscope which allows calcium imaging in awake, behaving animals across field-of-views (FOV) of up to 1.7 × 1.7 mm. A special scan system enables independent x,y, and z-positioning of two smaller sub-areas within this FOV for simultaneous functional recordings. This microscope enables us to optically record neuronal activity with cellular resolution across much larger spatial scales than previously possible and should help in deciphering the behavior-dependent flow of information within the neocortex. The microscope hard- and software are modular and can be extended to other imaging and photostimulation modalities.

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292C (5 March 2015); doi: 10.1117/12.2076388

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)