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Proceedings Paper

A modular two-photon microscope for simultaneous imaging of distant cortical areas in vivo
Author(s): Fabian F Voigt; Jerry L. Chen; Roland Krueppel; Fritjof Helmchen
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Paper Abstract

We have designed and built a two-photon microscope which allows calcium imaging in awake, behaving animals across field-of-views (FOV) of up to 1.7 × 1.7 mm. A special scan system enables independent x,y, and z-positioning of two smaller sub-areas within this FOV for simultaneous functional recordings. This microscope enables us to optically record neuronal activity with cellular resolution across much larger spatial scales than previously possible and should help in deciphering the behavior-dependent flow of information within the neocortex. The microscope hard- and software are modular and can be extended to other imaging and photostimulation modalities.

Paper Details

Date Published: 5 March 2015
PDF: 7 pages
Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292C (5 March 2015); doi: 10.1117/12.2076388
Show Author Affiliations
Fabian F Voigt, Univ. of Zürich (Switzerland)
Jerry L. Chen, Univ. of Zurich (Switzerland)
Roland Krueppel, Univ. of Zurich (Switzerland)
Deutsche Forschungsgemeinschaft (Germany)
Fritjof Helmchen, Univ. Zürich (Switzerland)

Published in SPIE Proceedings Vol. 9329:
Multiphoton Microscopy in the Biomedical Sciences XV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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