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Proceedings Paper

Multi-mode microscopy in real-time with LED array illumination
Author(s): Ziji Liu; Lei Tian; Laura Waller
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Paper Abstract

Brightfield, darkfield and phase contrast are three of the most popular imaging modalities in biological microscopy. Each provides visualization of different sample properties, without fluorescent labels or staining. We demonstrate here a single-camera imaging system that can simultaneously acquire brightfield, darkfield and phase contrast images in real-time. Our method uses a programmable LED array as the illumination source, which provides flexible patterning of illumination angles. We achieve a frame rate of 50 Hz with 2560×2160 pixels of lateral resolution, with speed being limited by the camera.

Paper Details

Date Published: 11 March 2015
PDF: 7 pages
Proc. SPIE 9336, Quantitative Phase Imaging, 93362M (11 March 2015); doi: 10.1117/12.2075951
Show Author Affiliations
Ziji Liu, Univ. of Electronic Science and Technology of China (China)
Univ. of California, Berkeley (United States)
Lei Tian, Univ. of California, Berkeley (United States)
Laura Waller, Univ. of California, Berkeley (United States)

Published in SPIE Proceedings Vol. 9336:
Quantitative Phase Imaging
Gabriel Popescu; YongKeun Park, Editor(s)

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