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Proceedings Paper

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane
Author(s): Rumelo Amor; Sumeet Mahajan; William B. Amos; Gail McConnell
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Paper Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

Paper Details

Date Published: 9 March 2015
PDF: 12 pages
Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 933010 (9 March 2015); doi: 10.1117/12.2075644
Show Author Affiliations
Rumelo Amor, Univ. of Strathclyde (United Kingdom)
Sumeet Mahajan, Univ. of Southampton (United Kingdom)
William B. Amos, MRC Lab. of Molecular Biology (United Kingdom)
Gail McConnell, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 9330:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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