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Proceedings Paper

Standardizing flow cytometric assays in long-term population-based studies
Author(s): Susanne Melzer; Jozsef Bocsi; Attila Tárnok
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Paper Abstract

Quantification of leukocyte subpopulations and characterization of antigen-expression pattern on the cellular surface can play an important role in diagnostics. The state of cellular immunology on the single-cell level was analyzed by polychromatic flow cytometry in a recent comparative study within the average Leipzig population (LIFE - Leipzig Research Centre for Civilization Diseases). Data of 1699 subjects were recorded over a long-time period of three years (in a total of 1126 days). To ensure compatibility of such huge data sets, quality-controls on many levels (stability of instrumentation, low intra-laboratory variance and reader independent data analysis) are essential. The LIFE study aims to analyze various cytometric pattern to reveal the relationship between the life-style, the environmental effects and the individual health. We therefore present here a multi-step quality control procedure for long-term comparative studies.

Paper Details

Date Published: 5 March 2015
PDF: 12 pages
Proc. SPIE 9315, Design and Quality for Biomedical Technologies VIII, 93150H (5 March 2015); doi: 10.1117/12.2075426
Show Author Affiliations
Susanne Melzer, Univ. Leipzig (Germany)
Jozsef Bocsi, Univ. Leipzig (Germany)
Attila Tárnok, Univ. Leipzig (Germany)
Translational Ctr. for Regenerative Medicine (Germany)

Published in SPIE Proceedings Vol. 9315:
Design and Quality for Biomedical Technologies VIII
Ramesh Raghavachari; Rongguang Liang, Editor(s)

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