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Proceedings Paper

Intracavity frequency doubling of a continuous wave Ti:sapphire ring laser and application in resonance Raman spectroscopy of heme protein dynamics
Author(s): Scott C. Buchter; Curtis Williams; Alfons Schulte; Theodore Alekel III; Gregory J. Mizell; William R. Fay
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Paper Abstract

Noncritical temperature-tuned phase-matching and large nonlinear coefficients make potassium niobate an attractive material for frequency doubling tuneable near-infrared radiation. We have mounted a KNbO3 crystal intracavity in an argon ion pumped, continuous wave Ti:Sapphire ring laser to increase the power level of the second harmonic. Wavelength selection at the fundamental frequency is accomplished with a birefringent filter. By using the crystal orientation that defines the d32 coefficient of KNbO3 we have obtained a blue second harmonic output tuneable from 425-445 nm. The laser is also characterized by the narrow linewidth of the Ti:Sapphire ring oscillator and good temporal stability. A continuous wave, frequency doubled Ti:sapphire laser is well suited to excite the resonance Raman spectrum in heme proteins with strong absorption bands in the range of 400 to 450 nm. We demonstrate the feasibility of such a setup for Raman studies of ligand binding to myoglobin. The Raman bands yield information on the reaction dynamics and on conformational changes near the linkage between the heme and the protein. In particular, a shift of the stretch frequency of the iron- histidine bond with high pressure may be attributed to a protein conformational change.

Paper Details

Date Published: 17 April 1995
PDF: 9 pages
Proc. SPIE 2380, UV and Visible Lasers and Laser Crystal Growth, (17 April 1995); doi: 10.1117/12.206943
Show Author Affiliations
Scott C. Buchter, Univ. of Central Florida (United States)
Curtis Williams, Univ. of Central Florida (United States)
Alfons Schulte, Univ. of Central Florida (United States)
Theodore Alekel III, Virgo Optics (United States)
Gregory J. Mizell, Virgo Optics (United States)
William R. Fay, Virgo Optics (United States)

Published in SPIE Proceedings Vol. 2380:
UV and Visible Lasers and Laser Crystal Growth
Richard Scheps; Milan R. Kokta, Editor(s)

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