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Proceedings Paper

Determination of nitric oxide mediating intracellular Ca2+ release on neurons based on confocal microscopy imaging
Author(s): Liqin Zheng; Yuhua Wang; Yipeng He; Yixiu Zeng; Yanding Zhang; Shusen Xie
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Paper Abstract

The gas NO is a ubiquitous intercellular messenger that modulates a wide range of physiological and pathophysiological functions. But few studies were made to study the role of NO in the Ca2+ release in dorsal root ganglion (DRG) neurons by confocal microscopy. Thus the objective of this study was to assess if NO has a role in Ca2+ signaling in DRG neurons using confocal microscopy combined with special fluorescence probe Fluo-3/AM. A 100 μM concentration of the NO donors (Sodium Nitroprusside, Dihydrate, SNP) and NO synthase inhibitor (NG-Monomethyl-L-arginine, Monoacetate salt, L-NMMA) was used in the study. Results showed that the fluorescence intensity increased rapidly after injecting SNP, which indicated that SNP could enhance intracellular Ca2+ release. And the fluorescence intensity shrank gradually with time and kept at a low level for quite a long period after loading with L-NMMA which indicated that L-NMMA could block intracellular Ca2+ release. All these results demonstrated that NO was involved in the regulation of intracellular Ca2+ release in the DRG neurons.

Paper Details

Date Published: 17 September 2014
PDF: 8 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301F (17 September 2014); doi: 10.1117/12.2068890
Show Author Affiliations
Liqin Zheng, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Yipeng He, Fujian Normal Univ. (China)
Yixiu Zeng, Fujian Normal Univ. (China)
Yanding Zhang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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