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Proceedings Paper

Serum albumin analysis for type II diabetes detection using surface-enhanced Raman spectroscopy
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Paper Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy combined with membrane electrophoresis (ME) was firstly employed to detect albumin variation in type II diabetic development. Albumin was first purified from human serum by ME and then mixed with silver nanoparticles to perform SERS spectral analysis. SERS spectra were obtained from blood albumin samples of 20 diabetic patients and 19 healthy volunteers. Subtle but discernible changes in the acquired mean spectra of the two groups were observed. Tentative assignment of albumin SERS bands indicated specific structural changes of albumin molecule with diabetic development. Meanwhile, PCA-LDA diagnostic algorithms were employed to classify the two kinds of albumin SERS spectra, yielding the diagnostic sensitivity of 90% and specificity of 94.7%. The results from this exploratory study demonstrated that the EM-SERS method in combination with multivariate statistical analysis has great potential for the label-free detection of albumin variation for improving type II diabetes screening.

Paper Details

Date Published: 17 September 2014
PDF: 7 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301Z (17 September 2014); doi: 10.1117/12.2068851
Show Author Affiliations
Jinyong Lin, Fujian Normal Univ. (China)
Gang Cao, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)
Nenrong Liu, Fujian Normal Univ. (China)
Fadian Liao, Fujian Normal Univ. (China)
Qiuyong Ruan, Fujian Normal Univ. (China)
Shanshan Wu, Fujian Normal Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Ling Li, The First Affiliated Hospital of Fujian Medical Univ. (China)
Rong Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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