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Proceedings Paper

Probability description of fluorescent and phosphorescent signal generation in tissues and other random media
Author(s): Eva Marie Sevick-Muraca; Christina L. Hutchinson
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Paper Abstract

The origin of fluorescent or phosphorescent signals re-emitted from highly scattering media (such as tissues) is investigated using a simple probability analysis developed earlier by Seveck et al. and adapted for incorporation of excited state probe kinetics. Results show that the lifetime of a uniformly distributed phosphorescent or fluorescent optical probe will profoundly affect the volume interrogated by a noninvasive, reflectance measurements. When the lifetime is greater than photon migration times, the origin of the re-emitted signal is confined closest to the surface. Our computations suggest that noninvasive measurements of tissue oxygen concentration may not necessarily interrogate deep tissues when systemically administered phosphorescent dyes are used. Instead, our results point to the development of short- lived probes and the successful deconvolution of nanosecond photon migration times for sensing subsurface conditions.

Paper Details

Date Published: 12 April 1995
PDF: 9 pages
Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); doi: 10.1117/12.206805
Show Author Affiliations
Eva Marie Sevick-Muraca, Purdue Univ. (United States)
Christina L. Hutchinson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 2387:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II
Robert R. Alfano, Editor(s)

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