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Proceedings Paper

Differentiation of bacterial colonies and temporal growth patterns using hyperspectral imaging
Author(s): Mehrube Mehrübeoglu; Gregory W. Buck; Daniel W. Livingston
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Paper Abstract

Detection and identification of bacteria are important for health and safety. Hyperspectral imaging offers the potential to capture unique spectral patterns and spatial information from bacteria which can then be used to detect and differentiate bacterial species. Here, hyperspectral imaging has been used to characterize different bacterial colonies and investigate their growth over time. Six bacterial species (Pseudomonas fluorescens, Escherichia coli, Serratia marcescens, Salmonella enterica, Staphylococcus aureus, Enterobacter aerogenes) were grown on tryptic soy agar plates. Hyperspectral data were acquired immediately after, 24 hours after, and 96 hours after incubation. Spectral signatures from bacterial colonies demonstrated repeatable measurements for five out of six species. Spatial variations as well as changes in spectral signatures were observed across temporal measurements within and among species at multiple wavelengths due to strengthening or weakening reflectance signals from growing bacterial colonies based on their pigmentation. Between-class differences and within-class similarities were the most prominent in hyperspectral data collected 96 hours after incubation.

Paper Details

Date Published: 19 September 2014
PDF: 15 pages
Proc. SPIE 9222, Imaging Spectrometry XIX, 922206 (19 September 2014); doi: 10.1117/12.2063601
Show Author Affiliations
Mehrube Mehrübeoglu, Texas A&M Univ. Corpus Christi (United States)
Gregory W. Buck, Texas A&M Univ. Corpus Christi (United States)
Daniel W. Livingston, Texas A&M Univ. Corpus Christi (United States)

Published in SPIE Proceedings Vol. 9222:
Imaging Spectrometry XIX
Pantazis Mouroulis; Thomas S. Pagano, Editor(s)

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