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Proceedings Paper

Optical tweezers escape forces
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Paper Abstract

With suitable calibration, optical tweezers can be used to measure forces. If the maximum force that can be exerted is of interest, calibration can be performed using viscous drag to remove a particle from the trap, typically by moving the stage. The stage velocity required to remove the particle then gives the escape force. However, the escape force can vary by up to 30% or more, depending on the particle trajectory. This can have significant quantitative impact on measurements. We describe the variation of escape force and escape trajectory, using both experimental measurements and simulations, and discuss implications for experimental measurement of forces.

Paper Details

Date Published: 16 September 2014
PDF: 7 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916413 (16 September 2014); doi: 10.1117/12.2062805
Show Author Affiliations
Ann A. M. Bui, The Univ. of Queensland (Australia)
Alexander B. Stilgoe, The Univ. of Queensland (Australia)
Nima Khatibzadeh, Univ. of California, Irvine (United States)
Timo A. Nieminen, The Univ. of Queensland (Australia)
Halina Rubinsztein-Dunlop, The Univ. of Queensland (Australia)
Michael W. Berns, Univ. of California, Irvine (United States)
Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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