In this presentation we report a new 3D scanned DSLM. The system combined 1) two-photon excitation, 2) scanning along the illumination axis (x-axis) using tunable acoustic gradient lens (TAG) to stretch the Rayleigh range [5], 3) scanning vertically to the illumination axis (y-axis) by one galvo mirror to create light sheet. 4) scanning along Z-axis to do fast 3D imaging by another galvo mirror. The image plane was kept aligned with the fast z-axis scanned light sheet plane by an electric tunable lens (ETL) as described in ref. 6. The light sheet can be tailored to any shape between 50×50 μm2 and more than 500×500 μm2 with constant thickness limited by diffraction and fast imaging rates limited by the detector. The tailorable illumination area allows multi-scale field of view (FOV), and is consequently capable of imaging cells, tissue and live animals in one setup.

Date Published: 5 September 2014
PDF: 6 pages
Proc. SPIE 9198, Ultrafast Nonlinear Imaging and Spectroscopy II, 91980W (5 September 2014); doi: 10.1117/12.2062690

Published in SPIE Proceedings Vol. 9198:
Ultrafast Nonlinear Imaging and Spectroscopy II
Zhiwen Liu, Editor(s)