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Proceedings Paper

Two-photon three-axis digital scanned light-sheet microscopy (2P3A-DSLM)
Author(s): Weijian Zong; Jia Zhao; Xuanyang Chen; Yuan Lin; Huixia Ren; Yunfeng Zhang; Ming Fan; Zhuan Zhou; Heping Cheng; Yujie Sun; Liangyi Chen
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Paper Abstract

In this presentation we report a new 3D scanned DSLM. The system combined 1) two-photon excitation, 2) scanning along the illumination axis (x-axis) using tunable acoustic gradient lens (TAG) to stretch the Rayleigh range [5], 3) scanning vertically to the illumination axis (y-axis) by one galvo mirror to create light sheet. 4) scanning along Z-axis to do fast 3D imaging by another galvo mirror. The image plane was kept aligned with the fast z-axis scanned light sheet plane by an electric tunable lens (ETL) as described in ref. 6. The light sheet can be tailored to any shape between 50×50 μm2 and more than 500×500 μm2 with constant thickness limited by diffraction and fast imaging rates limited by the detector. The tailorable illumination area allows multi-scale field of view (FOV), and is consequently capable of imaging cells, tissue and live animals in one setup.

Paper Details

Date Published: 5 September 2014
PDF: 6 pages
Proc. SPIE 9198, Ultrafast Nonlinear Imaging and Spectroscopy II, 91980W (5 September 2014); doi: 10.1117/12.2062690
Show Author Affiliations
Weijian Zong, Peking Univ. (China)
Institute of Basic Medical Sciences (China)
Jia Zhao, Peking Univ. (China)
Xuanyang Chen, Peking Univ. (China)
Yuan Lin, Peking Univ. (China)
Huixia Ren, Peking Univ. (China)
Yunfeng Zhang, Peking Univ. (China)
Ming Fan, Institute of Basic Medical Sciences (China)
Zhuan Zhou, Peking Univ. (China)
Heping Cheng, Peking Univ. (China)
Yujie Sun, Peking Univ. (China)
Liangyi Chen, Peking Univ. (China)

Published in SPIE Proceedings Vol. 9198:
Ultrafast Nonlinear Imaging and Spectroscopy II
Zhiwen Liu, Editor(s)

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