
Proceedings Paper
Targeting hepatocellular carcinoma with aptamer-functionalized PLGA/PLA-PEG nanoparticlesFormat | Member Price | Non-Member Price |
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Paper Abstract
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide, particularly in regions
where chronic Hepatitis B and C infections are common. Nanoparticle assemblies that incorporate high-affinity aptamers
which specifically bind malignant hepatocellular carcinoma cells could be useful for targeted drug delivery or enhancing
contrast with existing ablation therapies. The in vitro interactions of a tumor-specific aptamer, TLS11a, were
characterized in a hepatoma cell line via live-cell fluorescence imaging, SDS-PAGE and Western Blotting techniques.
Cell surface binding of the aptamer-AlexaFluor®546 conjugate was found to occur within 20 minutes of initial exposure,
followed by internalization and localization to late endosomes or lysosomes using a pH-sensitive LysoSensor™ Green
dye and confocal microscopy. Aptamer-functionalized polymer nanoparticles containing poly(lactic-co-glycolic acid)
(PLGA) and poly(lactide)-b-poly(ethylene glycol) (PLA-PEG) were then prepared by nanoprecipitation and passively
loaded with the chemotherapeutic agent, doxorubicin, yielding spherical nanoparticles approximately 50 nm in diameter.
Targeted drug delivery and cytotoxicity was assessed using live/dead fluorescent dyes and a MTT colorimetric viability
assay with elevated levels of cell death found in cultures treated with either the aptamer-coated and uncoated polymer
nanoparticles. Identification and characterization of the cell surface protein epitope(s) recognized by the TLS11a
aptamer are ongoing along with nanoparticle optimization, but these preliminary studies support continued investigation
of this aptamer and functionalized nanoparticle conjugates for targeted labeling and drug delivery within malignant
hepatocellular carcinomas.
Paper Details
Date Published: 27 August 2014
PDF: 10 pages
Proc. SPIE 9166, Biosensing and Nanomedicine VII, 916605 (27 August 2014); doi: 10.1117/12.2062283
Published in SPIE Proceedings Vol. 9166:
Biosensing and Nanomedicine VII
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)
PDF: 10 pages
Proc. SPIE 9166, Biosensing and Nanomedicine VII, 916605 (27 August 2014); doi: 10.1117/12.2062283
Show Author Affiliations
Shannon E. Weigum, Texas State Univ. (United States)
Melissa Sutton, Texas State Univ. (United States)
Eugenia Barnes, Texas State Univ. (United States)
Melissa Sutton, Texas State Univ. (United States)
Eugenia Barnes, Texas State Univ. (United States)
Sarah Miller, Texas State Univ. (United States)
Tania Betancourt, Texas State Univ. (United States)
Tania Betancourt, Texas State Univ. (United States)
Published in SPIE Proceedings Vol. 9166:
Biosensing and Nanomedicine VII
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)
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