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Proceedings Paper

Label free detection of phospholipids by infrared absorption spectroscopy
Author(s): Tahsin Ahmed; Erick Foster; Genevieve Vigil; Aamir A. Khan; Paul Bohn; Scott S. Howard
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Paper Abstract

We present our study on compact, label-free dissolved lipid sensing by combining capillary electrophoresis separation in a PDMS microfluidic chip online with mid-infrared (MIR) absorption spectroscopy for biomarker detection. On-chip capillary electrophoresis is used to separate the biomarkers without introducing any extrinsic contrast agent, which reduces both cost and complexity. The label free biomarker detection could be done by interrogating separated biomarkers in the channel by MIR absorption spectroscopy. Phospholipids biomarkers of degenerative neurological, kidney, and bone diseases are detectable using this label free technique. These phospholipids exhibit strong absorption resonances in the MIR and are present in biofluids including urine, blood plasma, and cerebrospinal fluid. MIR spectroscopy of a 12-carbon chain phosphatidic acid (PA) (1,2-dilauroyl-snglycero- 3-phosphate (sodium salt)) dissolved in N-methylformamide, exhibits a strong amide peak near wavenumber 1660 cm-1 (wavelength 6 μm), arising from the phosphate headgroup vibrations within a low-loss window of the solvent. PA has a similar structure to many important phospholipids molecules like phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylserine (PS), making it an ideal molecule for initial proof-of-concept studies. This newly proposed detection technique can lead us to minimal sample preparation and is capable of identifying several biomarkers from the same sample simultaneously.

Paper Details

Date Published: 27 August 2014
PDF: 7 pages
Proc. SPIE 9166, Biosensing and Nanomedicine VII, 91660H (27 August 2014); doi: 10.1117/12.2062094
Show Author Affiliations
Tahsin Ahmed, Univ. of Notre Dame (United States)
Erick Foster, Univ. of Notre Dame (United States)
Genevieve Vigil, Univ. of Notre Dame (United States)
Aamir A. Khan, Univ. of Notre Dame (United States)
Paul Bohn, Univ. of Notre Dame (United States)
Scott S. Howard, Univ. of Notre Dame (United States)

Published in SPIE Proceedings Vol. 9166:
Biosensing and Nanomedicine VII
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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