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Proceedings Paper

MG63 cells behavior on rough polypyrrole scaffolds investigated by digital holographic microscopy
Author(s): M. Mihailescu; A. Matei; A. Acasandrei; R. C. Popescu; I. A. Paun; Maria Dinescu
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Paper Abstract

The study of cells-substrate interaction became a stringent subject in the past decades, since an increasing variety of new materials and methods have been involved in tissue engineering or implants techniques. The investigation of this interaction using optical methods is a challenge, especially since these substrates are not optically polished. Due to their roughness in the micrometric or submicrometric range, the polymeric substrates offers good conditions for cells adhesion, but the characterization of cells properties can be hindered. In this study, we use Polypyrrole thin films, acting as substrates for cultured osteoblast-like MG63 cells having applications in tissue engineering for in vivo-like scaffolds. As characterization technique, we chose digital holographic microscopy, a single-shot technique, to obtain quantitative information about the sample features in a plane perpendicular to the substrate. Different parameters were tested in the experimental setup with the aim of finding the optimal conditions for details visualization. The reconstructed 3D images were filtered using a combination of analytical and implicit functions from MATLAB to exclude small/large objects, which correspond to Polypyrrole droplets to clearly identify the cells contour for quantitative measurements regarding their dimensions. These data were correlated with the effects on osteoblasts viability and differentiation. Also, the thickness and the refractive index of the substrate were determined using the decoupling procedure.

Paper Details

Date Published: 18 August 2014
PDF: 8 pages
Proc. SPIE 9204, Interferometry XVII: Advanced Applications, 92040N (18 August 2014); doi: 10.1117/12.2062016
Show Author Affiliations
M. Mihailescu, Univ. Politehnica of Bucharest (Romania)
A. Matei, National Institute for Lasers, Plasma and Radiation Physics (Romania)
A. Acasandrei, National Institute for Physics and Nuclear Engineering (Romania)
R. C. Popescu, Univ. Politehnica of Bucharest (Romania)
I. A. Paun, Univ. Politehnica of Bucharest (Romania)
National Institute for Lasers, Plasma and Radiation Physics (Romania)
Maria Dinescu, National Institute for Lasers, Plasma and Radiation Physics (Romania)

Published in SPIE Proceedings Vol. 9204:
Interferometry XVII: Advanced Applications
Cosme Furlong; Christophe Gorecki; Peter J. de Groot; Erik L. Novak, Editor(s)

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