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Proceedings Paper

Grating interferometry-based phase microtomography of atherosclerotic human arteries
Author(s): Marzia Buscema; Margaret N. Holme; Hans Deyhle; Georg Schulz; Rüdiger Schmitz; Peter Thalmann; Simone E. Hieber; Natalia Chicherova; Philippe C. Cattin; Felix Beckmann; Julia Herzen; Timm Weitkamp; Till Saxer; Bert Müller
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Paper Abstract

Cardiovascular diseases are the number one cause of death and morbidity in the world. Understanding disease development in terms of lumen morphology and tissue composition of constricted arteries is essential to improve treatment and patient outcome. X-ray tomography provides non-destructive three-dimensional data with micrometer-resolution. However, a common problem is simultaneous visualization of soft and hard tissue-containing specimens, such as atherosclerotic human coronary arteries. Unlike absorption based techniques, where X-ray absorption strongly depends on atomic number and tissue density, phase contrast methods such as grating interferometry have significant advantages as the phase shift is only a linear function of the atomic number. We demonstrate that grating interferometry-based phase tomography is a powerful method to three-dimensionally visualize a variety of anatomical features in atherosclerotic human coronary arteries, including plaque, muscle, fat, and connective tissue. Three formalin-fixed, human coronary arteries were measured using advanced laboratory μCT. While this technique gives information about plaque morphology, it is impossible to extract the lumen morphology. Therefore, selected regions were measured using grating based phase tomography, sinograms were treated with a wavelet-Fourier filter to remove ring artifacts, and reconstructed data were processed to allow extraction of vessel lumen morphology. Phase tomography data in combination with conventional laboratory μCT data of the same specimen shows potential, through use of a joint histogram, to identify more tissue types than either technique alone. Such phase tomography data was also rigidly registered to subsequently decalcified arteries that were histologically sectioned, although the quality of registration was insufficient for joint histogram analysis.

Paper Details

Date Published: 11 September 2014
PDF: 12 pages
Proc. SPIE 9212, Developments in X-Ray Tomography IX, 921203 (11 September 2014); doi: 10.1117/12.2060443
Show Author Affiliations
Marzia Buscema, Univ. Hospital Basel (Switzerland)
Margaret N. Holme, Univ. Hospital Basel (Switzerland)
Hans Deyhle, Univ. Hospital Basel (Switzerland)
Georg Schulz, Univ. Hospital Basel (Switzerland)
Rüdiger Schmitz, Univ. Hospital Basel (Switzerland)
Peter Thalmann, Univ. Hospital Basel (Switzerland)
Simone E. Hieber, Univ. Hospital Basel (Switzerland)
Natalia Chicherova, Univ. Hospital Basel (Switzerland)
Philippe C. Cattin, Univ. Hospital Basel (Switzerland)
Felix Beckmann, Helmholtz-Zentrum Geesthacht (Germany)
Julia Herzen, Helmholtz-Zentrum Geesthacht (Germany)
Technische Univ. München (Germany)
Timm Weitkamp, Synchrotron Soleil (France)
Till Saxer, Univ. Hospitals of Geneva (Switzerland)
Bert Müller, Univ. Hospital Basel (Switzerland)

Published in SPIE Proceedings Vol. 9212:
Developments in X-Ray Tomography IX
Stuart R. Stock, Editor(s)

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