Share Email Print

Proceedings Paper

Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA
Author(s): Mitchel J. Doktycz; K. Bruce Jacobson; K. L. Beattie; Robert S. Foote
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.

Paper Details

Date Published: 3 April 1995
PDF: 5 pages
Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206038
Show Author Affiliations
Mitchel J. Doktycz, Oak Ridge National Lab. (United States)
K. Bruce Jacobson, Oak Ridge National Lab. (United States)
K. L. Beattie, Houston Advanced Research Ctr. (United States)
Robert S. Foote, Oak Ridge National Lab. (United States)

Published in SPIE Proceedings Vol. 2386:
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
Gerald E. Cohn; Jeremy M. Lerner; Kevin J. Liddane; Alexander Scheeline; Steven A. Soper, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?