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Proceedings Paper

Scanning force microscopy study of native and linker histone-depleted chromatin fibers
Author(s): Guoliang Yang; Sanford S. Leuba; Carlos J. Bustamante; Kensal van Holde; Jordanka Zlatanova
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Paper Abstract

Scanning force microscopy has been used to study the structure of chromatin fibers at low salt concentrations. Chicken erythrocyte chromatin fibers in low ionic strength buffer solutions were deposited on mica and imaged in ambient conditions with a tapping mode scanning force microscope. Individual nucleosomes can be clearly discerned in the images of the fibers. Native chromatin fibers show an asymmetrical, 3D structure of sinuous fiber trajectory with irregularly positioned nucleosomes. Fibers depleted of linker histones H1 and H5 have a completely extended 'beads-on-a-string' structure, with linker DNA visible between single nucleosomes. Molecular modeling of the fiber architecture and computer simulation of the imaging process provided more evidence on the observed organization of chromatin at low salt conditions. These results have implications on mechanisms of transcription control and chromatin compaction.

Paper Details

Date Published: 30 March 1995
PDF: 9 pages
Proc. SPIE 2384, Scanning Probe Microscopies III, (30 March 1995); doi: 10.1117/12.205924
Show Author Affiliations
Guoliang Yang, Univ. of Oregon (United States)
Sanford S. Leuba, Univ. of Oregon (United States)
Carlos J. Bustamante, Univ. of Oregon (United States)
Kensal van Holde, Oregon State Univ. (United States)
Jordanka Zlatanova, Oregon State Univ. (United States)

Published in SPIE Proceedings Vol. 2384:
Scanning Probe Microscopies III
Mehdi Vaez-Iravani, Editor(s)

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