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Proceedings Paper

Imaging microtubules in buffer solution using tapping mode atomic force microscopy
Author(s): Monika Fritz; Manfred Radmacher; Miriam W. Allersma; Jason P. Cleveland; Russell J. Stewart; Paul K. Hansma; Christoph F. Schmidt
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Paper Abstract

Taxol stabilized microtubules were imaged in their native state in buffer solution without any fixation by an atomic force microscope (AFM) operated in tapping mode in liquids. Glass cover slips were derivatized with a positively charged silane to adsorb the filaments. The adsorbed microtubules could be imaged stably without any visible damage for hours. In cases where a microtubule crossed above another one, it often broke to follow the curvature of the underlying one. Longitudinal structures with a spacing of about 8 nm could be resolved suggesting they are protofilaments.

Paper Details

Date Published: 30 March 1995
PDF: 8 pages
Proc. SPIE 2384, Scanning Probe Microscopies III, (30 March 1995); doi: 10.1117/12.205923
Show Author Affiliations
Monika Fritz, Univ. of California/Santa Barbara (United States)
Manfred Radmacher, Univ. of California/Santa Barbara (United States)
Miriam W. Allersma, Univ. of Michigan (United States)
Jason P. Cleveland, Univ. of California/Santa Barbara (United States)
Russell J. Stewart, Univ. of Utah (United States)
Paul K. Hansma, Univ. of California/Santa Barbara (United States)
Christoph F. Schmidt, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 2384:
Scanning Probe Microscopies III
Mehdi Vaez-Iravani, Editor(s)

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