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Proceedings Paper

Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs
Author(s): Yu Wang; Altaz Khan; Madhura Som; Steven Y. Leigh; Danni Wang; Ye Chen; Patrick McVeigh; Brian C. Wilson; Jonathan T. C. Liu
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Paper Abstract

We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted to cell-surface biomarkers in fresh tissues. Ex vivo and in vivo experiments were performed to optimize our strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5 min) topical application of SERS NPs on tissues. The simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations, potentially enabling point-of-care surgical guidance or early disease detection.

Paper Details

Date Published: 19 May 2014
PDF: 3 pages
Proc. SPIE 9155, Translational Biophotonics, 91550Z (19 May 2014); doi: 10.1117/12.2057716
Show Author Affiliations
Yu Wang, Stony Brook Univ. (United States)
Altaz Khan, Stony Brook Univ. (United States)
Madhura Som, Stony Brook Univ. (United States)
Steven Y. Leigh, Stony Brook Univ. (United States)
Danni Wang, Stony Brook Univ. (United States)
Ye Chen, Stony Brook Univ. (United States)
Patrick McVeigh, Univ. of Toronto (Canada)
Princess Margaret Cancer Ctr. (Canada)
Brian C. Wilson, Univ. of Toronto (Canada)
Princess Margaret Cancer Ctr. (Canada)
Jonathan T. C. Liu, Stony Brook Univ. (United States)

Published in SPIE Proceedings Vol. 9155:
Translational Biophotonics
Tomasz S. Tkaczyk, Editor(s)

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