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Proceedings Paper

Measurement of 100 B. anthracis Ames spores within 15 minutes by SERS at the US Army Edgewood Chemical Biological Ctr.
Author(s): Stuart Farquharson; Chetan Shende; Wayne Smith; Hermes Huang; Jay Sperry; Todd Sickler; Amber Prugh; Jason Guicheteau
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Paper Abstract

Since the distribution of Bacillus anthracis-Ames spores through the US Postal System, there has been a persistent fear that biological warfare agents will be used by terrorists against our military abroad and our civilians at home. While there has been substantial effort since the anthrax attack of 2001 to develop analyzers to detect this and other biological warfare agents, the analyzers remain either too slow, lack sensitivity, produce high false-positive rates, or cannot be fielded. In an effort to overcome these limitations we have been developing a surface-enhanced Raman spectroscopy system. Here we describe the use of silver nanoparticles functionalized with a short peptide to selectively capture Bacillus anthracis spores and produce SER scattering. Specifically, measurements of 100 B. anthracis-Ames spores/mL in ~25 minutes performed at the US Army’s Edgewood Chemical Biological Center are presented. The measurements provide a basis for the development of systems that can detect spores collected from the air or water supplies with the potential of saving lives during a biological warfare attack.

Paper Details

Date Published: 5 May 2014
PDF: 8 pages
Proc. SPIE 9073, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV, 907315 (5 May 2014); doi: 10.1117/12.2054272
Show Author Affiliations
Stuart Farquharson, Real-Time Analyzers, Inc. (United States)
Chetan Shende, Real-Time Analyzers, Inc. (United States)
Wayne Smith, Real-Time Analyzers, Inc. (United States)
Hermes Huang, Real-Time Analyzers, Inc. (United States)
Jay Sperry, The Univ. of Rhode Island (United States)
Todd Sickler, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Amber Prugh, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Jason Guicheteau, U.S. Army Edgewood Chemical Biological Ctr. (United States)

Published in SPIE Proceedings Vol. 9073:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV
Augustus Way Fountain III, Editor(s)

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