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Proceedings Paper

Quantitative analysis of chromosome motion in Drosophila melanogaster
Author(s): Wallace Frank Marshall; David A. Agard; John W. Sedat
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Paper Abstract

We present an algorithm for estimating nonrigid motion of chromosomes in 4D microscopic images. Chromosomes are represented by a graph and motion estimation is formulated as a graph matching problem. All chromosomes within the graph are located, and then simulated annealing is used to find the mapping of chromosomes at time t onto chromosomes at time t+1 that minimizes the integrated displacement along each chromosome. Results with actual 4D images indicate that this model-based approach is sufficiently robust to correctly track the motion of chromosomes under conditions of limited spatial and temporal resolution. Using the motion estimate, previously developed methods for the quantitative analysis of 3D structure are extended to four dimensions, allowing chromosome mobility, flexibility, and interactions to be measured. Application of these algorithms to 4D images of Drosophila metaphase chromosomes in vivo allows visualization of clearly defined domains of high chromosomal flexibility, as well as other regions of distinctly lower chromosomal mobility which may coincide with centrometers.

Paper Details

Date Published: 23 March 1995
PDF: 10 pages
Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); doi: 10.1117/12.205345
Show Author Affiliations
Wallace Frank Marshall, Univ. of California/San Francisco (United States)
David A. Agard, Univ. of California/San Francisco (United States)
John W. Sedat, Univ. of California/San Francisco (United States)

Published in SPIE Proceedings Vol. 2412:
Three-Dimensional Microscopy: Image Acquisition and Processing II
Tony Wilson; Carol J. Cogswell, Editor(s)

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