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Proceedings Paper

Two-photon excited fluorescence microscopy combined with spectral and time-resolved measurements for fluorophore identification
Author(s): Ingrid Rokahr; Stefan Andersson-Engels
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Paper Abstract

Two-photon excited fluorescence microscopy was used to study unstained tissue and paper samples. As an excitation source a mode-locked Ti:Sapphire laser was utilized. In the experiments we used a conventional fluorescence microscope with a scanning board. The incoming laser pulses were focused onto the sample and the epifluorescence observed. In the spectroscopic measurements the fluorescence light was projected either on the slit of an polychromator with a CCD camera or, in some experiments, on a streak camera connected to the polychromator. The signal was then detected by a 2D-CCD camera. Fluorescence images were scanned by recording the fluorescence light pixel by pixel with a photomultiplier tube. Signal filtering and image processing were performed on a personal computer. Tissue samples from animals treated with photodynamic therapy were examined. The tissue contained protoporphyrin IX as a photosensitizer.

Paper Details

Date Published: 23 March 1995
PDF: 8 pages
Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); doi: 10.1117/12.205335
Show Author Affiliations
Ingrid Rokahr, Lund Institute of Technology (Sweden)
Stefan Andersson-Engels, Lund Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 2412:
Three-Dimensional Microscopy: Image Acquisition and Processing II
Tony Wilson; Carol J. Cogswell, Editor(s)

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