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Proceedings Paper

A microfluidic platform with integrated arrays for immunologic assays for biological pathogen detection
Author(s): Richard Klemm; Holger Becker; Nadine Hlawatsch; Sandra Julich; Peter Miethe; Christian Moche; Sebastian Schattschneider; Herbert Tomaso; Claudia Gärtner
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Paper Abstract

The ability to integrate complete assays on a microfluidic chip helps to greatly simplify instrument requirements and allows the use of lab-on-a-chip technology in the field. A core application for such field-portable systems is the detection of pathogens in a CBRN scenario such as permanent monitoring of airborne pathogens, e.g. in subway stations or hospitals etc. An immunological assay was chosen as method for the pathogen identification. The conceptual approach was its realization as a lab-on-a-chip system, enabling an easy handling of the sample in an automated manner. The immunological detection takes place on an antibody array directly implemented in the microfluidic network. Different immobilization strategies will be presented showing the performance of the system. Central elements of the disposable microfluidic device like fluidic interface, turning valves, liquid introduction and waste storage, as well as the architecture of measurement and control fluidic network, will be introduced. Overall process times of about 30 minutes were achieved and assays for the detection of Francisella tularensis and Yersinia pestis are presented. An important feature of the integrated lab-on-a-chip approach is that all waste liquids remain on-chip and contamination risks can be avoided.

Paper Details

Date Published: 29 May 2014
PDF: 7 pages
Proc. SPIE 9073, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV, 907311 (29 May 2014); doi: 10.1117/12.2050269
Show Author Affiliations
Richard Klemm, microfluidic ChipShop GmbH (Germany)
Holger Becker, microfluidic ChipShop GmbH (Germany)
Nadine Hlawatsch, microfluidic ChipShop GmbH (Germany)
Sandra Julich, Friedrich-Loeffler-Institut (Germany)
Peter Miethe, Forschungszentrum für Medizintechnik und Biotechnologie GmbH (Germany)
Christian Moche, microfluidic ChipShop GmbH (Germany)
Sebastian Schattschneider, microfluidic ChipShop GmbH (Germany)
Herbert Tomaso, Friedrich-Loeffler-Institut (Germany)
Claudia Gärtner, microfluidic ChipShop GmbH (Germany)

Published in SPIE Proceedings Vol. 9073:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XV
Augustus Way Fountain III, Editor(s)

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