
Proceedings Paper
Monitoring biofilm attachment on medical devices surfaces using hyperspectral imagingFormat | Member Price | Non-Member Price |
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Paper Abstract
Microbial biofilm is a colony of single bacteria cells (planktonic) that attached to surfaces, attract other microorganisms
to attach and grow, and together they build an extracellular matrix composed of polysaccharides, protein, and DNA.
Eventually, some cells will detach and spread to other surface. Biofilm on medical devices can cause severe infection to
all age ranges from infant to adult. Therefore, it is important to detect biofilm in a fast and efficient manner.
Hyperspectral imaging was utilized for distinguishing wide area of biofilm coverage on various materials and on
different textures of stainless steeltest coupons. Not only is the coverage of biofilm important, but also the shear stress of
biofilm on the attached surfaces is significant. This study investigates the effects of shear stress on the adhesion of
biofilms on common medical device surfaces such as glass, polycarbonate, polytetrafluoroethylene, and stainless steel
with different textures. Biofilm was grown using Ps. aeruginosa and growth was monitored after 24 and 48 hours at 37°
C. The coupons covered with biofilm were tilted at 45 degrees and 90 degrees for 30 seconds to induce shear stress and
Hyperspectral images were taken. We hypothesize that stronger attachment on rough surface would be able to withstand
greater shear stress compared to smooth surface.
Paper Details
Date Published: 20 February 2014
PDF: 10 pages
Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893809 (20 February 2014); doi: 10.1117/12.2047867
Published in SPIE Proceedings Vol. 8938:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV
Israel Gannot, Editor(s)
PDF: 10 pages
Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893809 (20 February 2014); doi: 10.1117/12.2047867
Show Author Affiliations
Hanh N. D. Le, U.S. Food and Drug Administration (United States)
Johns Hopkins Univ. (United States)
Victoria M Hitchins, U.S. Food and Drug Administration (United States)
Johns Hopkins Univ. (United States)
Victoria M Hitchins, U.S. Food and Drug Administration (United States)
Ilko K. Ilev, U.S. Food and Drug Administration (United States)
Do-Hyun Kim, U.S. Food and Drug Administration (United States)
Do-Hyun Kim, U.S. Food and Drug Administration (United States)
Published in SPIE Proceedings Vol. 8938:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV
Israel Gannot, Editor(s)
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