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Proceedings Paper

Delivery of tobramycin coupled to iron oxide nanoparticles across the biofilm of mucoidal Pseudonomas aeruginosa and investigation of its efficacy
Author(s): Leisha M. Armijo; Michael Kopciuch; Zuzia Olszόwka; Stephen J. Wawrzyniec; Antonio C. Rivera; John B. Plumley; Nathaniel C. Cook; Yekaterina I. Brandt; Dale L. Huber; Gennady A. Smolyakov; Natalie L. Adolphi; Hugh D. C. Smyth; Marek Osiński
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Paper Abstract

Pseudomonas aeruginosa bacterium is a deadly pathogen, leading to respiratory failure in cystic fibrosis and nosocomial pneumonia, and responsible for high mortality rates in these diseases. P. aeruginosa has inherent as well as acquired resistance to many drug classes. In this paper, we investigate the effectiveness of two classes; aminoglycoside (tobramycin) and fluoroquinolone (ciprofloxacin) administered alone, as well as conjugated to iron oxide (magnetite) nanoparticles. P. aeruginosa possesses the ability to quickly alter its genetics to impart resistance to the presence of new, unrecognized treatments. As a response to this impending public health threat, we have synthesized and characterized magnetite nanoparticles capped with biodegradable short-chain carboxylic acid derivatives conjugated to common antibiotic drugs. The functionalized nanoparticles may carry the drug past the mucus and biofilm layers to target the bacterial colonies via magnetic gradient-guided transport. Additionally, the magnetic ferrofluid may be used under application of an oscillating magnetic field to raise the local temperature, causing biofilm disruption, slowed growth, and mechanical disruption. These abilities of the ferrofluid would also treat multi-drug resistant strains, which appear to be increasing in many nosocomial as well as acquired opportunistic infections. In this in vitro model, we show that the iron oxide alone can also inhibit bacterial growth and biofilm formation.

Paper Details

Date Published: 8 April 2014
PDF: 12 pages
Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 89550I (8 April 2014); doi: 10.1117/12.2043340
Show Author Affiliations
Leisha M. Armijo, The Univ. of New Mexico (United States)
Michael Kopciuch, The Univ. of New Mexico (United States)
Zuzia Olszόwka, The Univ. of New Mexico (United States)
Albuquerque Institute for Math and Science (United States)
Stephen J. Wawrzyniec, The Univ. of New Mexico (United States)
Antonio C. Rivera, The Univ. of New Mexico (United States)
John B. Plumley, The Univ. of New Mexico (United States)
Nathaniel C. Cook, The Univ. of New Mexico (United States)
Yekaterina I. Brandt, The Univ. of New Mexico (United States)
Dale L. Huber, Sandia National Labs. (United States)
Gennady A. Smolyakov, The Univ. of New Mexico (United States)
Natalie L. Adolphi, The Univ. of New Mexico (United States)
Hugh D. C. Smyth, The Univ. of Texas at Austin (United States)
Marek Osiński, The Univ. of New Mexico (United States)
Univ. Carlos III de Madrid (Spain)

Published in SPIE Proceedings Vol. 8955:
Colloidal Nanoparticles for Biomedical Applications IX
Wolfgang J. Parak; Marek Osinski; Kenji I. Yamamoto M.D., Editor(s)

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