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Proceedings Paper

Efficacy of photodynamic therapy against larvae of Aedes aegypti: confocal microscopy and fluorescence-lifetime imaging
Author(s): L. M. de Souza; S. Pratavieira; N. M. Inada; C. Kurachi; J. Corbi; F. E. G. Guimarães; V. S. Bagnato
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Paper Abstract

Recently a few demonstration on the use of Photodynamic Reaction as possibility to eliminate larvae that transmit diseases for men has been successfully demonstrated. This promising tool cannot be vastly used due to many problems, including the lake of investigation concerning the mechanisms of larvae killing as well as security concerning the use of photosensitizers in open environment. In this study, we investigate some of the mechanisms in which porphyrin (Photogem) is incorporated on the Aedes aegypti larvae previously to illumination and killing. Larvae at second instar were exposed to the photosensitizer and after 30 minutes imaged by a confocal fluorescence microscope. It was observed the presence of photosensitizer in the gut and at the digestive tract of the larva. Fluorescence-Lifetime Imaging showed greater photosensitizer concentration in the intestinal wall of the samples, which produces a strong decrease of the Photogem fluorescence lifetime. For Photodynamic Therapy exposition to different light doses and concentrations of porphyrin were employed. Three different light sources (LED, Fluorescent lamp, Sun light) also were tested. Sun light and fluorescent lamp shows close to 100% of mortality after 24 hrs. of illumination. These results indicate the potential use of photodynamic effect against the LARVAE of Aedes aegypti.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89472D (4 March 2014); doi: 10.1117/12.2040420
Show Author Affiliations
L. M. de Souza, Univ. de São Paulo (Brazil)
Federal Univ. de São Carlos (Brazil)
S. Pratavieira, Univ. de São Paulo (Brazil)
N. M. Inada, Univ. de São Paulo (Brazil)
C. Kurachi, Univ. de São Paulo (Brazil)
Federal Univ. de São Carlos (Brazil)
J. Corbi, Univ. de São Paulo (Brazil)
F. E. G. Guimarães, Univ. de São Paulo (Brazil)
V. S. Bagnato, Univ. de São Paulo (Brazil)
Federal Univ. de São Carlos (Brazil)

Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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